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sbp hibit il1β  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sbp hibit il1β
    Sbp Hibit Il1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sbp+hibit+il1%CE%B2/IL-1%CE%B2/pmc12908108-134-82-111
    Average 96 stars, based on 2283 article reviews
    sbp hibit il1β - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Plasmid Preparation:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Saline:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Stable Transfection:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Expressing:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Biomarker Discovery:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Construct:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use

    Low Protein Binding:

    Article Title: Protocols for Monitoring Unconventional Protein Secretion Using Luminescence and Trapping Approaches
    Article Snippet: .. HEK293T cells (ATCC CRL‐3216) Complete growth medium for HEK293 cells (see recipe) with and without P/S Opti‐MEM reduced‐serum medium (Gibco, cat. no. 31985‐062) TransIT‐2020 transfection reagent (Mirus, cat. no. MIR5400) Plasmids: pCDH‐Str‐KDEL (ER lumen‐facing hook; Addgene, cat. no.65307) pCDH‐Str‐Ii (ER cytosolic‐facing hook; Addgene, cat. no. 65313) psPAX lentiviral packaging plasmid (Addgene, cat. no. 12260) pMD2.G VSV‐G envelope plasmid (Addgene, cat. no. 12259) Dulbecco's phosphate‐buffered saline (DPBS) without Ca 2+ /Mg 2+ (Sigma‐Aldrich, cat. no. D8537) SH‐SY5Y cells stably expressing SBP‐HiBiT‐Tau, SBP‐HiBit‐TNFα, or SBP‐HiBit‐IL1β (see Support Protocol ) Complete growth medium for SH‐SY5Y cells (see recipe) with and without P/S Polybrene (Millipore, cat. no. TR‐1003‐G) Puromycin (Sigma‐Aldrich, cat. no. P9620) Anti‐streptavidin antibody (Santa Cruz Biotechnology, cat. no. sc‐52236) for validation of hook construct Biotin (Sigma‐Aldrich, cat. no. B4639) 10‐cm tissue culture dishes (Corning, cat. no. 353003) 37°C, 5% CO 2 incubator 0.45‐μm low protein‐binding filter (VWR, cat. no. 28145‐479) .. 89 ml DMEM (Sigma‐Aldrich, cat. no. D6429) 10 ml heat‐inactivated fetal bovine serum (FBS, Gibco, cat. no.10270‐106) 1 ml penicillin‐streptomycin (P/S; Gibco, cat. no. 15140‐122) Mix thoroughly Store up to 2 weeks at 4°C Warm to 37°C before use



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    sbp hibit il1β  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology sbp hibit il1β
    Sbp Hibit Il1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sbp+hibit+il1%CE%B2/IL-1%CE%B2/pmc12908108-134-82-111
    Average 96 stars, based on 1 article reviews
    sbp hibit il1β - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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